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KMID : 0604520140400010011
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Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 1 p.11 ~ p.20
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Antioxidative Effect and Active Component Analysis of Gnaphalium affine D. DON. Extracts
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Kim Hae-Soo
Im Na-Ri
Park Jun-Ho
Kim Myeong-Ok
Park Soo-Nam
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Abstract
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In this work, the antioxidative effects and active component analysis of Gnaphalium affine D. DON. (G. affine)extracts were investigated. All experiments were performed with 70% ethanol extract, ethyl acetate fraction and aglyconefraction of the G. affine extract. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50) ofethyl acetate fraction (6.15 μg/mL) of the G. affine was higher than that of (+)-α-tocopherol (8.89 μg/mL), whichis known as a reference control. Reactive oxygen species (ROS) scavenging activities (OSC50) of the 70% ethanol extract(1.60 μg/mL), ethyl acetate fraction (0.075 μg/mL) and aglycone fraction (2.28 μg/mL) of extract of G. affine onROS generated in Fe3+-EDTA/H2O2 system using the luminol-dependent chemiluminescence assay were much higherthan that of L-ascorbic acid (6.88 μg/mL). The cellular protective effects of 70% ethanol extract (τ50 = 52.0 min)and aglycone fraction of the extract (τ50 = 60.6 min) on the 1O2-induced cellular damage of human erythrocytes wereexhibited the higher protective effect than (+)-α-tocopherol (τ50 = 38.0 min), known as a lipophilic antioxidant. TLCand HPLC were used to analyse active components in the aglycone fraction of the extract. Results showed that luteolin and apigenin were main components. These results suggest that the G. affine extract can be applied to an effective antioxidantin scavenging ROS including radicals.
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KEYWORD
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Gnaphalium affine D. DON., antioxidative activity, flavonoid, photoaging
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